Electrode plates are fully separated to prevent arcing or damage. This unique design feature enables several gels to be stacked and. The upper electrode housing is separate from the base allowing it to be secured with hand screws at the desired height. Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. Semi-Dry Blotter with transfer area dimensions: 20cm x 20cm The stainless steel cathode and platinum-coated titanium anode transfer gels reliably and quickly and are durable and corrosion-free. The Semi-Dry Blotter plates are graphite coated to reduce heat, minimize buffer usage and produce clear transfer bands that are not distorted. Set-up procedures are easy and economical with little buffer needed. Blotting is fast and easy, and can typically be completed in 15 to 30 min. Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. The kuroGEL semi-dry blotter provides great flexibility in a laboratory since it can be used for all types of blotting: Western, Southern and Northern. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use. Cut 6 pieces of Extra Thick blotting paper and a piece of blotting membrane to the exact size of the gel to be blotted. These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. Due to very low buffer volumes typically only a few millilitres of buffer are required per transfer. The iBlot 3 Transfer Stack consists of a Bottom Stack and a Top Stack sandwiching a pre-run gel and a nitrocellulose (0. Semi Dry Blotting has the added benefit of economic transfers. The iBlot 3 Western Blot Transfer System is based on the dry blotting concept, utilizing a unique gel matrix technology. Each unit is compatible with their respective omniPAGE vertical gel system. When PVDF membranes are used, however, we recommend supplementation with SDS (0.01-0.05 ) in all cases, since blotting efficiency onto PVDF is significantly enhanced in the presence of SDS. All units can be used for all types of blotting: Western, Southern and Northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Several stacks of transfer units can be run simultaneously.These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting typically 15 to 30 minutes. The time required to complete the transfer is dependent on molecule size and the number of transfer units being transferred. The bands from the charged molecules of the gels migrate and bind to the membrane to complete the transfer. An electric current is applied perpendicular to the gel and buffer saturated filter papers. The IB44000 and IB45000 semi dry blotters have plates that are graphite coated to reduce heat, minimize buffer usage and produce clear transfer bands that are not distorted.Ī transfer unit stack is formed by the gel and membrane being in contact and sandwiched between filter papers soaked in transfer buffer. Ideal for efficient and economic transfer of protein, RNA, or DNA from polyacrylamide or agarose gels to a solid membrane support, which is subsequently visualized by staining methods.
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